Scientific Publications


Andrea Aebischer, Kerstin Wernike, Patricia Konig, Kati Franzke, Paul J. Wichgers Schreur, Jeroen Kortekaas, Marika Vitikainen, Marilyn Wiebe, Markku Saloheimo, Ronen Tchelet, Jean-Christophe Audonnet, Martin Beer: Click here for the link to the paper in Vaccines MDPI


van Gool, M.P., van Muiswinkel, G.C.J., Hinz, S.W.A., Schols, H.A., Sinitsyn, A.P., Gruppen, H. “Two novel GH11 endo-zylanases from Myceliophthora thermophila C1 act differently toward soluble and insoluble xylans”.Enzyme and Microbial Technology 53 (2013) 25-32.

Koutaniemi, S., van Gool, M.P., Juvonen, M., Jokela, J., Hinz, S.W., Schols, H.A., Tenkanen, M. “Distinct roles of carbohydrate esterase family CE16 acetyl esterases and polymer-acting acetyl xylan esterases in xylan deacetylation.”Journal of Biotechnology 168 (2013) 684-692.


van Gool, M.P., van Muiswinkel, G.C.J., Hinz, S.W.A., Schols, H.A.,  Sinitsyn, A.P.,  Gruppen, H. “Two GH10 endo-xylanases of Myceliophthora thermophila C1 with and without cellulose binding module act differently towards soluble and insoluble substrates”. Bioresource Technology 2012: doi: 10.1016/j.biotech.2012.05.117.

Klyosov, A.A., Dotsenko, G.S., Hinz, S.W.A., and Sinitsyn, A.P. “Structural features of β-(1>4)-D-galactomannans of plant origin as a probe for β-(1>4)- mannanase polymeric substrate specificity.”Carbohydrate Research May 2012: 352:65-69.

Dotsenko, G.S., Sinitsyn, O.A., Hinz, S.W.A., Wery, J. and Sinitsyn, A.P. “Characterization of a GH family 3 b-glycoside hydrolase from Chrysosporium lucknowense and its application to the hydrolysis of b-glucan and xylan.”Bioresource Technology May 2012: 112:345–349.

Kühnel, S., Pouvreau, L., Appeldoorn, M.M., Hinz, S.W.A, Schols, H.A. and Gruppen, H. “The Ferulic Acid Esterases of Chrysosporium Lucknowense C1: Purification, Characterization and Their Potential Application in Biorefinery.” Enzyme and Microbial Technology January 2012: 5;50(1):77-85.


Berka, Randy et al. “Comparative genomic analysis of the thermophilic biomass-degrading fungi Myceliophthora thermophila and Thielavia terrestris.” Nature Biotechnology October 2011.

Punt, Peter J., Levasseur, Anthony, Visser, Hans, Wery, Jan and Record, Eric. “Fungal Protein Production: Design and Production of Chimeric Proteins.” The Annual Review of Microbiology October 2011: 65: 57–56.

Visser, Hans, Joosten, Vivi, Punt, Peter J., Gusakov, Alexander V., Olson, Phil T., Joosten, Rob, Bartels, Jeffrey, Visser, Jaap, Sinitsyn, Arkady P., Emalfarb, Mark A., Verdoes, Jan C., and Wery, Jan.  “Development of a mature fungal technology and production platform for industrial enzymes based on a Myceliophthora thermophila isolate, previously known as Chrysosporium lucknowense C1.” Industrial Biotechnology June 2011.

Pouvreau, L., Jonathan, M.C., Kabel, M.A., Hinz, S.W., Gruppen, H. and Schols, H.A. “Characterization and mode of action of two acetyl xylan esterases from Chrysosporium lucknowense C1 active towards acetylated xylans.” Enzyme and Microbial Technology August 2011: 49(3):312-320.

Pouvreau, Laurice, Joosten, Rob, Hinz, Sandra W.A., Gruppen, Harry and Schols, Henk A. “Chrysosporium lucknowense C1 arabinofuranosidases are selective in releasing arabinose from either single or double substituted xylose residues in arabinoxylans.” Enzyme and Microbial Technology April 2011: 7; 48(4-5):397-403.

Kühnel, S., Westphal, Y., Hinz, S.W.A, Schols, H.A. and Gruppen, H. “Mode of action of Chrysosporium lucknowense C1 a-L-arabinohydrolases.” Bioresource Technology January 2011: 102(2):1636-1643.

Gusakov, A.V.  “Alternatives to Trichoderma reesei in biofuel production.”  Trends in Biotechnology, 2011, v.29, No. 9, p.419-425


Kühnel, S., Hinz, S.W.A., Pouvreau, L., Wery, J., Schols, H.A. and Gruppen, H. “Chrysosporium lucknowense arabinohydrolases effectively degrade sugar beet arabinan.” Bioresource Technology November 2010: 101(21):8300-8307.

Westphal, Y., Kühnel, S., de Waard, P., Hinz, S.W.A., Schols, H.A., Voragen, A.G. and Gruppen, H. “Branched arabino-oligosaccharides isolated from sugar beet arabinan.” June 2010: 345(9):1180-1189.

Kühnel, S., Hinz, S.W.A., Pouvreau, L., Wery, J., Schols, H.A. and Gruppen, H. “Chrysosporium lucknowense arabinohydrolases effectively degrade sugar beet arabinan.” Bioresource Technology November 2010: 101(21):8300-8307.


Hinz, S.W., Pouvreau, L., Joosten, R., Bartels, J., Jonathan, M.C., Wery, J. and Schols, H.A. “Hemicellulase production in Chrysosporium lucknowense C1.” Journal of Cereal Science 2009: 50(3): 318–323.

Gusakov, A.V.,Ustinov, B.B.  “Assaying sensitivity of fungal xylanases to proteinaceous inhibitors from a rye extract: two GH10 xylanases resistant to XIP-like inhibitors.”  Industrial Biotechnology, 2009, v.5, No. 2, p.104-109


Gusakov, A.V., Antonov, A.I. and Ustinov, B.B. “N-Glycosylation in Chrysosporium lucknowense enzymes.” January 2008: 343(1):48-55.

Ustinov, B.B., Gusakov, A.V., Antonov, A.I., Sinitsyn, A.P.  “Comparison of properties and mode of action of six secreted xylanases from Chrysosporium lucknowense.”  Enzyme and Microbial Technology, 2008, v.43, No. 1, p.56-65


Gusakov, A.V., Salanovich, T.N., Antonov A.I., Ustinov, B.B, Okunev, O.N., Burlingame, R., Emalfarb, M., Baez, M. and Sinitsyn, A.P. “Design of highly efficient cellulase mixtures for enzymatic hydolysis of cellulose.” Biotechnol Bioengineering August 2007: 97(5):1028-1038.

Verdoes, J.C., Punt, P.J., Burlingame, R.P., Bartels, J., van Dijk, R., Slump, E., Meens, M., Joosten, R. and Emalfarb, M. “A dedicated vector for efficient library construction and high throughput screening in the hyphal fungus Chrysosporium lucknowense.” Industrial Biotechnology Spring 2007: 48-57.


Sanderson, Katherine. “A Field in Ferment.” Nature, December 2006: Vol. 444.

Burlingame, R.P. and Verdoes, J.C. “Maximizing Protein Expression in Filamentous Fungi.” BioPharm International May 2006: 19(5):40-47.

Saha, Badal C. and Cotta, Michael A. “Ethanol Production from Alkaline Peroxide Pretreated Enzymatically Saccharified Wheat Straw.” Biotechnology Prog. January 2006: 22,449-453.


Burlingame, R.P. and Chandra R. “Gene discovery and protein production technology: An integrated system to discover, develop and manufacture enzymes and other proteins.” Industrial Biotechnology 2005: 1(1):35-37.

Gusakov, A.V., Sinitsyn, A.P., Salanovich, T.N., Bukhtojarov, F.E., Markov, A.V., Ustinov, B.B., van Zeijl, C., Punt, P., Burlingame, R.  “Purification, cloning and characterisation of two forms of thermostable and highly active cellobiohydrolase I (Cel7A) produced by the industrial strain of Chrysosporium lucknowense.”  Enzyme and Microbial Technology, 2005, v.36, No. 1, p.57-69


Bukhtojarov, F.E., Ustinov, B.B., Salanovich, T.N., Antonov, A.I., Gusakov, A.V., Okunev, O.N. and Sinitsyn, A.P. “Cellulase Complex of the Fungus Chrysosporium lucknowense: Isolation and Characterization of Endoglucanases and Cellobiohydrolases. Biochemistry (Moscow) May 2004: 69(5):542-551.

Potera, Carol, “From stone-washed jeans to gene expression.” Genetic Engineering News August 2004: 24(14).


Burlingame R.P., Kozlowski M.R. and Lightbody B.G. “An Integrated Approach to Protein Development.” Pharmagenomics March/April 2003.


Gusakov, A.V., Sinitsyn, A.P., Markov, A.V., Sinitsyna, O.A., Ankudimova, N.V.,Berlin, A.G.  “Study of protein adsorption on indigo particles confirms the existence of enzyme-indigo interaction sites in cellulase molecules.” Journal of Biotechnology, 2001, v.87, No. 1, p.83-90

Sinitsyn, A.P., Gusakov, A.V., Grishutin, S.G., Sinitsyna, O.A., Ankudimova, N.V.  “Application of microassays for investigation of cellulase abrasive activity and backstaining.”  Journal of Biotechnology, 2001, v.89, No. 2, p.233-238


Gusakov, A.V., Sinitsyn, A.P.,Berlin, A.G., Markov, A.V., Ankudimova, N.V. “Surface hydrophobic amino acid residues in cellulase molecules as a structural factor responsible for their high denim-washing performance.”  Enzyme and Microbial Technology, 2000, v.27, No. 9, p.664-671


Gusakov, A.V., Sinitsyn, A.P.,Berlin, A.G., Popova, N.N., Markov, A.V., Okunev, O.N., Tikhomirov, D.F., Emalfarb, M. “Interaction between indigo and adsorbed protein as a major factor causing backstaining during cellulase treatment of cotton fabrics.”  Applied Biochemistry and Biotechnology, 1998, v.75, p.279-293